研究業績（熊本大学免疫学講座）

Gao T, Irie A, Kouwaki T, Oshiumi H.

Development of a single-chain variable antibody fragment against a conserved region of the SARS-CoV-2 spike protein.

Scientific Reports 14: 14419, 2024

Yamaguchi M, Mtali YS, Sonokawa H, Takashima K, Fukushima Y, Kouwaki T, Oshiumi H.

HPV vaccines induce trained immunity and modulate pro-inflammatory cytokine expression in response to secondary Toll-like receptor stimulations.

Microbiology and Immunology 68: 65-74, 2024

Baba Y, Yasuda N, Bundo M, Nakachi Y, Ueda J, Ishimoto T, Iwatsuki M, Miyamoto Y, Yoshida N, Oshiumi H, Iwamoto K, Baba H.

LINE-1 hypomethylation, increased retroposition and tumor-specific insertion in upper gastrointestinal cancer.

Cancer Science 115: 247-256, 2024

Matsunaga, T, Sano H, Takita K, Morita M, Yamasaki S, Ichikawa T, Numakura T, Ida T, Jung M, Ogata S, Yoon S, Fujino N, Kyogoku Y, Sasaki Y, Koarai A, Tamada T, Toyoma A, Nakabayashi T, Kageyama L, Kyuwa S, Inabe K, Watanabe S, Nagy P, Sawa T, Oshiumi H, Ichinose M, Yamada M, Sugiura H, Wei FY, Motohashi H, Akaike T..

Supersulphides provide airway protection in viral and chronic lung diseases.

Nature Communications 14: 4476, 2023

Toyoda M, Tan TS, Motozono C, Barabona G, Yonekawa A, Shimono N, Minami R, Nagasaki Y, Miyashita Y, Oshiumi H, Nakamura K, Matsushita S, Kuwata T, Ueno T.

Evaluation of neutralizing activity against omicron subvariants in BA.5 breakthrough infection and three-dose vaccination using a novel chemiluminescence-based, virus-mediated cytopathic assay.

Microbiology Specctrum 11: e0066023, 2023

Kouwaki T, Nishimura T, Wang G, Nakagawa R, Oshiumi H.

K63-linked polyubiquitination of LGP2 by Riplet regulates RIG-I-dependent innate immune response

EMBO Reports 24:(2) e54844, 2023

Miyashita Y, Yoshida T, Takagi Y, Tsukamoto H, Takashima K, Kouwaki T, Makino K, Fukushima S, Nakamura K, Oshiumi H. 

Circulating extracellular vesicle microRNAs associated with adverse reactions, proinflammatory cytokine, and antibody production after COVID-19 vaccination

NPJ Vaccines 7: 16, 2022

Tsukamoto H, Komohara Y, Tomita Y, Miura Y, Motoshima T, Imamura K, Kimura T, Ikeda T, Fujiwara Y, Yano H, Kamba T, Sakaguchi T, Oshiumi H.

Aging-associated and CD4 T-cell-dependent ectopic CXCL13 activation predispose to anti-PD-1 therapy-induced adverse events.

PNAS 119: e2205378119, 2022

Iwamoto A, Tsukamoto H, Nakayama H, Oshiumi H.

E3 ubiquitin ligase Riplet is expressed in T cells and suppresses T cell-mediated antitumor immune responses. 

Journal of Immunology 208: 2067-2076, 2022

Oka K, Fujioka S, Kawamura Y, Komohara Y, Chujo T, Sekiguchi K, Yamamura Y, Oiwa Y, Omamituda-ishikawa N, Komaki S, Sutoh Y, Sakurai S, Tomizawa K, Bono H, Shimizu A, Araki K, Yamamoto T, Yamada Y, Oshiumi H, Miura K.

Resistance to chemical carcinogenesis induction via a dampened inflammatory response in naked mole-rats.

Communications Biology 5: 287, 2022

Tsutsuki H. Zhang T, Yahir K, Ono K, Fujiwara Y, Iyoda S, Wei FY, Monde K, Seto K, Ohnishi M, Oshiumi H, Akaike T, Sawa T.

Subtilase cytotoxin from Shiga-toxigenic Escherichia coli impairs the inflammasome and exacerbates enteropathogenic bacterial infection. 

iScience 25: 104050, 2022

Nagayoshi Y, Nishiguchi K, Yamamura R, Chujo T, Oshiumi H, Nagata H, Kaneko H, Yamoto K, Nakata H, Sakakida K, Kunisawa A, Adachi M, Kakizoe Y, Mizobe T, Kuratsu JI, Shimada S, Nakamori Y, Matsuoka M, Mukoyama M, Wei FY, Tomizawa K.

T6A and Ms2t6 A modified nucleosides in serum and urine as strong candidate biomarkers of COVID1-19 infection and severity.

Biomolecules 12: 1244, 2022

Motozono C, Toyoda M, Tan TS, Hamana H, Goto Y, Aritsu Y, Miyashita Y, Oshiumi H, Nakamura K, Okada S, Udaka K, Kitamatsu M, Kishi H, Ueno T. 

The SARS-CoV-2 Omicoron BA.1 spike G446S mutation potentiates antiviral T-cell recognition. 

Nature Communications 12: 5440, 2022

Miyashita Y, Kouwaki T, Tsukamoto H, Okamoto M, Nakamura K, Oshiumi H.

TICAM-1/TRIF associates with Act1 and suppresses IL-17 receptor-mediated inflammatory responses.

Life Science Alliance 5: e202101181, 2021

Fukuda H, Chujo T, Wei FY, Shi SL, Hirayama M, Kaitsuka T, Ymamoto T, Oshiumi H, Tomizawa K.

Cooperative methlation of human tRNALys3 at position A58 and U54 drives the early and late step of HIV replication.

Nucleic Acid Res. 49: 11855-11867, 2021

Shi SL, Fukuda H, Chujo T, Kouwaki T, Oshiumi H, Tomizawa K, Wei FY.

Export of RNA-derived modified nucleosides by equilibrative nucleoside transporters defines the magnitude of autophagy rsponse and Zika virus replication.

RNA Biology. 18: 478-495, 2021

Kouwaki T, Nishimura T, Wang G, Oshiumi H 

RIG-I-like receptor-mediated recognition of viral genomic RNA of severe acute respiratory syndrome coronavirus-2 and viral escape from the host innate immune responses

Frontiers in Immunology 12: 700926

Oshiumi H.

Circulating Extracellular Vesicles Carry Immune Regulatory miRNAs and Regulate Vaccine Efficacy and Local Inflammatory Response After Vaccination.

Frontiers in Immunology 12: 685344, 2021

Nakashima M, Ishikawa K, Fugiwara A, Shu K, Fukushima Y, Okamoto M, Tsukamoto H, Kouwaki T, Oshiumi H.

miR-451a levels rather than human papillomavirus vaccine administration is associated with severity of murine experimental autoimmune encephalomyelitis.

Scientific Reports 11: 9369, 2021

Tsukamoto H, Komohara Y, Oshiumi H.

The role of macrophages in anti-tumor immune responses: pathological significance and potential as therapeutic targets.

Human Cell 34: 1031-1039, 2021

Imamura R, Kitagawa S, Kubo T, Irie A, Kariu T, Yoneda M, Kamba T, Imamura T.

Prostate cancer C5a receptor expression and augmentation of cancer cell proliferation, invasion, and PD-L1 expression by C5a

The Prostate 81: 147-156, 2021

Tsukamoto H, Kouwaki T, Oshiumi H.

Aging-associated extracellular vesicles contain Immune regulatory microRNAs alleviating hyperinflammatory state and immune dysfunction in the elderly

iScience 2020 23: 101520

Oshiumi H.

Recent advances and contradictions in the study of the individual roles of ubiquitin ligases that regulate RIG-I-like receptor-mediated antiviral innate immune responses

Frontiers in immunology 2020 11:1296

Wang G, Kouwaki T, Mugikura K, Okamoto M, Takaki H, Funami K, Seya T, Oshiumi H. 

Cytoplasmic dsRNA induces the expression of OCT3/4 and NANOG mRNAs in differentiated human cells

J Biol. Chem. 294: 18969-18979. 2019

Wang G, Kouwaki T, Okamoto M, Oshiumi H

Attenuation of the Innate Immune Response against Viral Infection Due to ZNF598-Promoted Binding of FAT10 to RIG-I

Cell Reports 28: 1961-1970. 2019

Miyashita M, Ishikawa K, Fukushima Y, Kouwaki T, Nakamura K, Oshiumi H.

Immune-regulatory microRNA expression levels within circulating extracellular vesicles correspond with the appearance of local symptoms after seasonal flue vaccination.

PLoS One 14: e0219510. 2019

Okamoto M, Fukushima Y, Kouwaki T, Daito T, Kohara M, Kida H, Oshiumi H

MicroRNA-451a in extracellular, blood-resident vesicles attenuates macrphage and dendritic cell responses to influenza whole-virus vaccine.

J. Biol. Chem. 293: 18585-18600. 2018

Tsukamoto H, Fujieda K, Miyashita A, Fukushima S, Ikeda T, Kubo Y, Senju S, Ihn H, Nishimura Y, Oshiumi H

Combined blockade of IL-6 and PD-1/PD-L1 signaling abrogates mutual regulation of their immunosuppressive effects in the tumor environment. 

Cancer Research 78: 5011-5022. 2018

Fukushima Y, Okamoto M, Ishikawa K, Kouwaki T, Tsukamoto H, Oshiumi H

Activation of TLR3 and its adaptor TICAM-1 increases miR-21 levels in extracellular vesicles released from human cells. 

BBRC 500: 744-750, 2018

Takashima K, Oshiumi H, Matsumoto M, Seya T

TICAM-1 is dispensable in STING-mediated innate immune responses in myeloid immune cells

BBRC 499: 985-991, 2018

Okamoto M, Kouwaki T, Fukushima Y, Oshiumi H

Regulation of RIG-I Activation by K63-Linked Polyubiquitination

Frontiers in Immunology 8:1942, 2018

Takashima K, Oshiumi H, Matsumoto M, Seya T

DNAJB1/HSP40 Suppresses Melanoma Differentiation-Associated Gene 5-Mitochodrial Antiviral Signaling Protein function in Conjunction with HSP70.

J. Innate Immunity 10: 44-55, 2018

Tsukamoto H, Fujieda K, Senju S, Ikeda T, Oshiumi H, Nishimura Y

Immune-suppressive effects of IL-6 on T-cell-mediated anti-tumor immunity.

Cancer Science  10: 44-55, 2018

Fujikura D, Muramatsu D, Toyomane K, Chiba S, Daito T, Iwai A, Kouwaki T, Okamoto M, Higashi H, Kida H, Oshiumi H

Aureobasidium pullulans-cultured fluid induces IL-18 production, leading to Th1-polarization during influenza A virus infection.

J. Biochem 163: 31-38, 2018

Kouwaki T, Okamoto M, Tsukamoto H, Fukushima Y, Matsumoto M, Seya T, Oshiumi H

Zyxin stabilizes RIG-I and MAVS interactions and promotes type I interferon response.

Scientific Reports 7: 11905, 2017

Okamoto M, Tsukamoto H, Kouwaki T, Seya T, Oshiumi H

Recognition of viral RNA by pattern recognition receptors in the induction of innate immunity and excessive inflammation during respiratory viral infections.

Viral Immunology 30: 408-420, 2017

Takaki H, Oshiumi H, Shingai M, Matsumoto M, Seya T.

Development of mouse models for analysis of human virus infections

Microbiology and Immunology 61: 107-113, 2017

Kouwaki T, Okamoto M, Tsukamoto H, Fukushima Y, and Oshiumi H

Extracellular vesicles deliver host and virus RNA and regulate innate immune resonse

International Journal of Molecular Sciences 18: 666, 2017

Shibata Y, Tokunaga F, Goto E, Komatsu G, Gohda J, Saeki Y, Tanaka K, Takahashi H, Sawasaki T, Inoue S, Oshiumi H, Seya T, Nkano H, Tanaka Y, Iwai K, and Inoue JI.

HTLV-1 Tax induces Formation of the Acitive Macromolecular IKK Complex by Generating Lys63- and Met1-Linked Hybrid Polyubiquitin Chains

PLoS Pathog. 13: e1006162, 2017 

Leong CR, Funami K, Oshiumi H, Mangao D, Takaki H, Matsumoto M, Aly HH, Watashi K, Chayama K, and Seya T.

Interferon-stimulated gene of 20 kDa protein (ISG20) degrades RNA of hepatitis B virus to impede the replication of HBV in vitro and in vivo.

Oncotarget 7: 68149-68193, 2016

Takashima K, Takeda Y, Oshiumi H, Shime H, Okabe M, Ikawa M, Matsumoto M, and Seya T.

STING in tumor and host cells cooperatively work for NK cell-mediated tumor growth retardation.

BBRC 478: 1764-1771, 2016

Funami K, Matsumoto M, Oshiumi H, Obuse C, and Seya T.

The dataset of proteins specifically interacted with activated TICAM-1.

Data Brief 8: 697-699, 2016

Azuma M, Takeda Y, Nakajima H, Sugiyama H, Ebihara T, Oshiumi H, Matsumoto M, and Seya T.

Biphasic function of TLR3 adjuvant on tumor and spleen dendritic cells promotes tumor T cell infiltration and regression in a vaccine therapy. Oncoimmunology 5: e1188244, 2016

Kouwaki T, Fukushima Y, Daito T, Sanada T, Yamamoto N, Mifsud EJ, Leong CR, Tsukiyama-Kohara K, Kohara M, Matsumoto M, Seya T, and Oshiumi H. Extracellular vesicles including exosomes regulate innate immune response to hepatitis B virus infection. Frontiers in Immunology 7: 335 eCollection 2016 

Kasamatsu J, Deng M, Azuma M, Funami K, Shime H, Oshiumi H, Matsumoto M, Kasahara M, Seya T.

Double-stranded RNA analog and type I interferon regulate expression of Trem paired receptors in murine myeloid cells. BMC Immunol. 17(1):9. 2016

Oshiumi H, Kouwaki T, Seya T.

Accessory Factors of Cytoplasmic Viral RNA Sensors Required for Antiviral Innate Immune Response. Frontiers in Immunology 7: 200 eCollection 2016 

Oshiumi H, Mifsud EJ, Daito T. Links between recognition and degradation of cytoplasmic viral RNA in innate immune response. Reviews in Medical Virology: 26: 90-101, 2016 

*Oshiumi H, Miyashita M, Okamoto M, Morioka Y, Okabe M, Seya T. DDX60 is involved in RIG-I-dependent and –independent antiviral responses and its function is attenuated by virus-induced EGF receptor activation. Cell Reports: 11: 1193-1207. 2015 *Corresponding author

Takashima K, *Oshiumi H, Takaki H, Matsumoto M, Seya T.　RIOK3-mediated phosphorylation of MDA5 interferes with its assembly and attenuates the innate immune response. Cell Reports 11: 192-200. 2015. *Corresponding author

Oshiumi H*, Miyashita M, Matsumoto M, and Seya T.  A Distinct Role of Riplet-Mediated K63-Linked Polyubiquitination of the RIG-I Repressor Domain in Human Antiviral Innate Immune Responses.  PLoS Pathogens 9, e1003533 (2013)

Miyashita M, Oshiumi H*, Matsumoto M, and Seya T.  DDX60, a DExD/H box helicase, is a novel antiviral factor promoting RIG-I-like receptor mediated signaing. Mol. Cell. Biol.　31, 3802-3819 (2011) *Corresponding author

Oshiumi H*, Miyashita M, Inoue N, Okabe M, Matsumoto M, and Seya T. The ubiquitin ligase Riplet is essential for RIG-I-dependent innate immune responses to RNA virus infection. Cell Host & Microbe 8, 496-509 (2010)

Oshiumi H, Matsumoto M, Hatakeyama S, Seya T. Riplet/RNF135, a RING finger protein, ubiquitinates RIG-I to promote interferon-beta induction during the early phase of viral infection. J Biol Chem. 284, 807-817 (2009)

Hayase A, Takagi M, Miyazaki T, Oshiumi H, Shinohara M, and Shinohara A. A protein complex containing Mei5 and Sae3 promotes the assembly of the meiosis-specific RecA homolog Dmc1. Cell 119, 927-940 (2004)

Oshiumi H, Sasai M, Shida K, Fujita T, Matsumoto M, Seya T* TICAM-2: a bridging adapter recruiting to Toll-like receptor 4 TICAM-1 that induces interferon-beta. J Biol Chem. 278, 49751-49762 (2003) 

Oshiumi H, Matsumoto M, Funami K, Akazawa T, and Seya T* 

TICAM-1, an adapter molecule that participates in Toll-like receptor 3-mediated interferon-beta induction.  Nature Immunology 4, 161-167 (2003)

Oshiumi H, Tsujita T, Shida K, Matsumoto M, Ikeo K, and Seya T* 

Prediction of the prototype of the human Toll-like receptor gene family from the pufferfish Fugu rubripes genome.  Immunogenetics  54, 791-800 (2003)

Usui T^#, Ohta T^#, Oshiumi H^#, Tomizawa J, Ogawa H, and Ogawa T..

Complex Formation and Functional Versatility of Mre11 of Budding Yeast in Recombination.  Cell 95, 705-716 (1998) ^#These authors contributed equally to this work.